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KMID : 0892920210300040308
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Experimental Neurobiology
2021 Volume.30 No. 4 p.308 ~ p.317
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Attenuation of Experimental Autoimmune Uveitis in Lewis Rats by Betaine
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Choi Yu-Na
Jung Kyung-Sook
Kim Hyo-Jin
Chun Ji-Yoon
Ahn Mee-Jung
Jee Young-Heun
Ko Hyun-Ju
Moon Chang-Jong
Matsuda Hiroshi
Tanaka Akane
Kim Jeong-Tae
Shin Tae-Kyun
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Abstract
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Experimental autoimmune uveitis (EAU) is an animal model of human autoimmune uveitis that is characterized by the infiltration of autoimmune T cells with concurrent increases in pro-inflammatory cytokines and reactive oxygen species. This study aimed to assess whether betaine regulates the progression of EAU in Lewis rats. EAU was induced via immunization with the interphotoreceptor retinoid-binding protein (IRBP) and oral administration of either a vehicle or betaine (100 mg/kg) for 9 consecutive days. Spleens, blood, and retinas were sampled from the experimental rats at the time of sacrifice and used for the T cell proliferation assay, serological analysis, real-time polymerase chain reaction, and immunohistochemistry. The T cell proliferation assay revealed that betaine had little effect on the proliferation of splenic T cells against the IRBP antigen in an in vitro assay on day 9 post-immunization. The serological analysis showed that the level of serum superoxide dismutase increased in the betainetreated group compared with that in the vehicle-treated group. The anti-inflammatory effect of betaine was confirmed by the downregulation of pro-inflammation-related molecules, including vascular cell adhesion molecule 1 and interleukin-1β in the retinas of rats with EAU. The histopathological findings agreed with those of ionized calcium-binding adaptor molecule 1 immunohistochemistry, further verifying that inflammation in the retina and ciliary bodies was significantly suppressed in the betaine-treated group compared with the vehicle-treated group. Results of the present study suggest that betaine is involved in mitigating EAU through anti-oxidation and anti-inflammatory activities.
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KEYWORD
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Anti-inflammation, Anti-oxidation, Betaine, Experimental autoimmune uveitis, Retina
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